Establishment of a confluent cardiomyocyte culture in a cylindrical microchannel

Abstract

We established a confluent cardiomyocyte culture method using an 800-μm diameter cylindrical microchannel in this report. This was realized by introducing cardiomyocytes 2 times before and after turning over a microchip. The optimum condition was starting the flowing medium 2.0 h after seeding and flowing the medium at 1.0 μL/min. By applying this technology to a cardiomyocyte-based spherical heart pump device, one may develop self-fluid regulated devices that could be applied for implantable or circulation analysis device on a chip. © 2011 The Japan Society for Analytical Chemistry.