Unusual Regulatory Properties of An Halophilic Aspartate Aminotransferase

Abstract

An experimental system has been devised for the optimum aerobic growth and induction of the halophilic aspartate aminotransferase of an archaebacterium Haloferax mediterranei incubated in the presence of laspartate. H. mediterranei utilizes both L-aspartate and yeast extract as a sole or combined energy source. The maximum synthesis of the enzyme was promoted by 1 % L-aspartate. The relationship between holo and apo forms of the halophilic transaminase, as the mechanism of the induction system, seems to be related to unusual regulatory properties. © 1991, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation. All rights reserved.