Use of Mesalazine for the Determination of Dopamine and Its Pharmaceutical Preparations by Spectrophotometric Method

Abstract

A sensitive spectrophotometric method was used for the determination of Dopamine (DM) in a basic medium via its oxidative coupling by Mesalazine (MES), which was exploited as a reagent and Na2S2O8 as an oxidizing agent, the measurement was at a wavelength of 522 nm. The proposed method was successfully applied for the determination of dopamine (DM) in the pharmaceutical preparation (ampules). It was valid within a concentration range of 2.7 31.25 μg.mL-1 and the linear regression was R2 = 0.9977. The important analytical parameter include: molar absorptivity (0.45×104 l.mol-1.cm-1), the Sandell's sensitivity index (0.03343 μg/cm2), limit of detection (LOD) and limit of quantification (LOQ) were (0.37 and 1.23 μg.ml-1), respectively. It also succeeded in giving acceptable ttest values.