A new phytoecdysteroid from the stem bark of Vitex cienkowskii

Abstract

To investigate differences in the metabolome of the stem bark of Vitex cienkowskii harvested at three different locations and three different seasons, their extracts were analyzed by means of UPLC–ESI–IMS–TOF MSe. One marker metabolite was isolated and chemically characterized, which was identified as the new compound 20,24-dihydroxy,24-hydroxymethylecdysone (1). IMS showed two drift time species for 1 which could be used as new and additional characteristic compound parameters in compound identification to reduce dereplication and false positives.