Elimination of mRNA splicing by a point mutation outside the conserved GU at 5′ splice sites

Abstract

Nearly all mRNA introns begin with the dinucleotide GU. Mutations in either of these virtually invariant bases have been found to inactivate the corresponding 5′ splice site. Until now single base changes in neighboring bases have not been found to completely inactivate a 5′ splice site. Here we show that a single A→11 transversion in the third position of the adenovirus? F1A 13S mRNA intron does prevent RNA splicing at the corresponding 5′ splice site. © 1984 IRL Press Limited, Oxford, England.