Cryopreservation of Gaga’ chicken semen from South Sulawesi, Indonesia with the addition of L-carnitine, hyaluronic acid, sucrose and their combination in diluent

Abstract

Khaeruddin, Wahjuningsih S, Ciptadi G, Yusuf M, Hermawansyah, Sahiruddin. 2022. Cryopreservation of Gaga’ chicken semen from South Sulawesi, Indonesia with the addition of L-carnitine, hyaluronic acid, sucrose and their combination in diluent. Biodiversitas 23: 3297-3302. Gaga' chicken (Gallus gallus domesticus) is Indonesian germplasm that needs to be preserved with cryopreservation technology. Therefore, this study aims to determine the effect of adding L-Carnitine, hyaluronic acid, and sucrose as well as their combination in a diluent on the quality of Gaga' chicken spermatozoa after freeze-thawing. A completely randomized design was used along with 6 treatments, namely P0, P1, P2, P3, P4, and P5, containing Ringer acetate-egg yolk (RAEY) diluent (P0), RAEY + 0.063 mM hyaluronic acid (P1), RAEY + 1 mM L-carnitine (P2), RAEY + 1 mM sucrose (P3), RAEY + 0.063 mM hyaluronic acid + 1 mM L-carnitine (P4), and RAEY + 0.063 mM hyaluronic acid + 1 mM L-carnitine + 1mM sucrose (P5). Liquid semen was packed in a 0.25 mL straw, equilibrated at 5oC for 2 hours, and placed 3 cm above the surface of liquid nitrogen for 10 minutes. It was then immersed in nitrogen for 24 hours and thawed at 60°C for 5 seconds. The results showed that there was a significant difference (P<0.05) in the motility, viability, and kinematic variables of spermatozoa after freeze-thawing. P1, P2, P3, and P4 increased progressive motility, P1 and P4 increased curvilinear velocity, while viability increased with P4 treatment. Furthermore, the addition of Hyaluronic acid, L-carnitine, or their combination in the RAEY diluent can improve the sperm quality of Gaga' chickens.