Optimization of treatments for the germination and establishment of botanical yam (Dioscorea rotundata) seeds

Abstract

Botanical yam (Dioscorea rotundata) seeds are currently used by yam breeders to generate and conserve new varieties of yam instead of seed yams (vegetative propagule). Yam seed treatment through scarification is one of the most effective methods of improving seed germination and seedling establishment. This study was carried out under greenhouse condition to determine the best seed treatment for the germination and establishment of botanical yam seeds. The set up involved a soil mixed with carbonized rice husk using five different seed treatments: dry heating (DHS) at 60°C, mechanical scarification + gibberellic acid (MSS + GA), mechanical scarification (MSS), acid scarification with sulfuric acid (ASS) and soaking in hot water at 80°C (SOS). The findings showed that the treatment, MSS + GA and MSS only, had significant (p<0.05) positive effect on the seed emergence and seedling establishment compared to the untreated control (COS) and other treatments. ASS caused seed damage resulting to no emergence. The findings showed that carbonized grain waste (rice husk) enhanced soil fertility and seed treatment (MSS + GA) was the best to improve yam seed germination and seedling establishment. These treatments can be efficiently applied to reduce the long gestation periods and increase yield in yam breeding programs.