Inhibitory effects of proanthocyanidins isolated from barley bran on hyaluronidase activity, soybean lipoxygenase activity and complementary activity

Abstract

The effects of proanthocyanidin (dimer and trimer) isolated from BPE (a polyphenol extract from barley bran) on hyaluronidase activity, soybean lipoxygenase activity and complementary activity in vitro were studied in comparison with catechins. Inhibition to these activities was depended on BPE concentration. Inhibition of proanthocyanidins to hyaluronidase was more effective than that of (+)-catechin, (-)-epicatechin and (-)-epigallocatechin, however, less effective than that of (-)-epigallocatechin gallate ((-)-EGCG) and (-)-epicatechin gallate ((-)-ECG). The inhibitory effects of proanthocyanidins on lipoxygenase activity were found to be higher than those of catechins. The anti-complementary activities of proanthocyanidins except procyanidin B 3 were found to be same or more effective than those of (-)-EGCG and (-)-ECG. These results suggested that BPE and proanthocyanidins isolated from BPE might be good inhibitors to inflammation and allergic reaction.