Chemical oxidative polymerization of conductive polyaniline-iron oxide composite as an electro-transducer for electrochemical sensing applications

Abstract

This study explored the preparation of conductive polyaniline-iron oxide (PANI-Fe2O3) that served as the electrical signal transducer, to convert the electrochemical interactions between the biotinylated-goat-anti-mouse IgG (b-IgG) and bovine serum albumin (BSA) into a measurable resistance signal. In this study, PANI was synthesized through the oxidative polymerization of aniline monomer (AM) in the presence of ammonium persulfate (APS) as the oxidizing agent. Concentration effects of AM and volume ratios of AM:APS were evaluated so as to obtain higher conductivity performance in an electrochemical sensing application. The synthesized PANI composites were analyzed through conductivity measurement. The conductive PANI-Fe2O3-GA-b-IgG bio-conjugates was then interacted with BSA, relays the antigen-antibody binding as a measured electrical detection in an assembled pulse-mode electrochemical biosensor. In present work, 0.2 m of AM with 1:3 volume ratio of AM:APS has contributed to the optimal ionic conductivity property of PANI, with excellent electrochemical sensing performance recorded at 3.538±0.067 mΩ.