Analysis of Paralytic Shellfish Poison and Tetrodotoxin by Ion-Pairing High Performance Liquid Chromatography

Abstract

An ion-pairing high performance liquid chromatographic method using a silica ODS column was developed for analysis of paralytic shellfish poison (PSP) and tetrodotoxin (TTX). As mobile phase is used a mixture of 0.05 m phosphate buffer (pH 7.0) containing 2 mM heptanesulfonic acid/methanol (99:1) for separation of gonyautoxins and TTXs, and the one (75: 25) for separation of saxitoxins. PSP and TTX members thus separated were detected as fluorogenic substances, by heating with a periodate reagent (for PSP), or with 3 n naoh (for TTX). © 1987, The Japanese Society of Fisheries Science. All rights reserved.