Co-transfer of B cells converts resistance into susceptibility in T cell-reconstituted, Leishmania major-resistant C.B-17 scid mice by a non-cognate mechanism

Abstract

Resistance to infection of mice with Leishmania major parasites is dependent on the production of IFN-γ by CD4+ T helper cells. C.B-17 acid mice, lacking both T and B cells, succumb very quickly to the infection, but develop resistance if reconstituted with appropriate numbers of T cells from BALB/c mice. In this model, we studied the role of B cells with regard to their ability to influence disease outcome and to function as antigen-presenting cells for T cells. For this purpose, we reconstituted acid mice (H-2(d)) with either T cells or with T and B cells obtained from (BALB/c x BALB.B)F1 mice (H-2(d x b)), and infected them with L. major parasites 1 day after reconstitution. Mice reconstituted with T cells alone cured the disease, whereas additional B cell reconstitution led to susceptibility. Healing was associated with a predominant T(h)1-type response. In all mice, L. major-specific T cell proliferation was restricted to the MHC phenotype of the recipient (H-2(d)) but not to that of the donor (H-2(d x b)), indicating that there was no detectable contribution of donor B cells in the priming of a T cell response. Furthermore, B cells, when purified from infected BALB/c mice, were unable to stimulate a L. major-specific CD4+ T cell clone (L1/1) without addition of exogenous antigen, in contrast to macrophages from the same animal. These data suggest that B cells, in vivo, do not carry L. major antigen in a form capable of activating specific CD4+ T cells. Therefore, B cells promote disease by means other than cognate interaction with CD4+ T cells.