Effect of various methods of removing non-specific inhibitors to virus hemagglutination upon serum proteins and immunoglobulins

Abstract

The effects of various commonly used procedures for removing non-specific viral inhibitors upon serum proteins and immunoglobulins were evaluated. Several types of effects were observed. Treatment with ether, chicken red blood cells, receptor destroying enzyme (RDE), and RDE plus heat (65‡ C/30 minutes) resulted in a generalized increase in the immunoglobulin concentration. Conversely, treatment with RDE plus mercaptoethanol and with periodate produced a generalized decrease in immunoglobulin concentration. The significance of the findings upon interpretation of serological results is discussed. © 1971 Springer-Verlag.