INCREASING THE EFFICIENCY OF BACTERIOLOGICAL DIAGNOSIS OF UREGENITAL TRICHOMONIASIS USING THE IMPROVED NUTRIENT MEDIUM

Abstract

The scientific and practical significance of the problem of urogenital trichomoniasis is determined by the prevalence and variability of the pathogen. It is assumed that under adverse conditions, these protozoa lose the ability to move intensively and undergo morphological transformation. The existence of such a problem generates the scientific and practical interest in the matter of improvement of bacteriological methods of diagnosis when examining patients with chronic inflammatory processes of the urogenital tract. The purpose of the study - optimization of the composition of the nutrient medium for bacteriological detection of the trichomoniasis pathogen, considering the existence of different morphotypes and ways of trichomonas’ motility. 50 culture samples of Trichomonas vaginalis, taken from 293 patients with chronic diseases of the genitourinary system, served as the object of study, in their comparative cultivation in the improved and standard environment using the methods of classical bacteriology. It has been evidentiated that the optimal ratio of additional ingredients, tested in the course of the experiments, has led to the increased growth and increased biomass of the pathogen, indicating the improvement in the quality of the nutrient medium. The data obtained indicates that the changed components of the optimized nutrient medium have improved the quality of the diagnostic procedure especially in patients with a torpid course of the disease.