Mutation K42R in ribosomal protein S12 does not affect susceptibility of mycobacterium smegmatis 16s rRNA a-site mutants to 2-deoxystreptamines

Abstract

Recent studies have suggested that ribosomal protein S12 modulates 16S rRNA function and susceptibility to 2- deoxystreptamine aminoglycosides. To study whether the non-restrictive K42R mutation in RpsL affects 2-deoxystreptamine susceptibility in Mycobacterium smegmatis, we studied the drug susceptibility pattern of various mutants with genetic alterations in the 16S rRNA decoding A-site in the context of wild-type and mutant protein S12. RpsL K42R substitution was found not to affect the drug resistance pattern associated with mutational alterations in 16S rRNA H44. © 2010 Kalapala et al.