Modulation of Mg 2+ influx and cytoplasmic free Mg 2+ concentration in rat ventricular myocytes

Abstract

To examine whether TRPM7, a member of the melastatin family of transient receptor potential channels, is a physiological pathway for Mg 2+ entry in mammalian cells, we studied the effect of TRPM7 regulators on cytoplasmic free Mg 2+ concentration ([Mg 2+ ] i ) of rat ventricular myocytes. Acutely isolated single cells were AM-loaded with the fluorescent indicator furaptra, and [Mg 2+ ] i was estimated at 25 °C. After [Mg 2+ ] i was lowered by soaking the cells with a high-K + and Mg 2+ -Ca 2+ -free solution, [Mg 2+ ] i was recovered by extracellular perfusion of Ca 2+ -free Tyrode’s solution that contained 1 mM Mg 2+ . The initial rate of increase in [Mg 2+ ] i was analyzed as the Mg 2+ influx rate. The Mg 2+ influx rate was increased by the TRPM7 activator, naltriben (2–50 μM), in a concentration-dependent manner with a half maximal effective concentration (EC 50 ) of 24 μM. This EC 50 value is similar to that reported for the activation of recombinant TRPM7 overexpressed in HEK293 cells. Naltriben (50 μM) caused little change in basal [Mg 2+ ] i (~ 0.9 mM) in Ca 2+ -free Tyrode’s solution, but significantly raised [Mg 2+ ] i to 1.31 ± 0.03 mM in 94 min after the removal of extracellular Na + . Re-introduction of extracellular Na + lowered [Mg 2+ ] i back to the basal level even in the presence of naltriben. Application of 10 μM NS8593, an inhibitor of TRPM7, significantly lowered [Mg 2+ ] i to 0.72 ± 0.03 mM in 50-60 min independent of extracellular Na + . The results suggest that Mg 2+ entry through TRPM7 significantly contributes to physiological Mg 2+ homeostasis in mammalian heart cells.