Lumpy skin disease virus identification in different tissues of naturally infected cattle and chorioallantoic membrane of emberyonated chicken eggs using immunofluorescence, immunoperoxidase techniques and polymerase chain reaction

Abstract

Lumpy Skin Disease Virus (LSDV) was detected in 23 samples collected from clinically diseased and slaughtered cattle showed clinical signs believed to be LSD. These samples include 7 skin lesions and 16 internal organs (lymph nodes (6), lung (4), kidney (3) and liver (3). Hyperimmune serum was prepared against reference LSDV (Ismailyia 88 strain). Immunofluorescence (IF), Immunoperoxidase (IP) techniques and Polymerase Chain Reaction (PCR) were used in our study. Chorioallantoic Membranes (CAMs) of Embryonated Chicken Eggs (ECEs) were inoculated with Known previously isolated and identified LSDV of 10 48 EID eo/0.1 mL for virus follow up in these CAMs by using IF and IP techniques and PCR. The results indicate that IF and IP techniques are useful in the quick diagnosis of the disease in naturally infected cattle. PCR could be used for rapid and specific detection of LSDV nucleic acid in crude skin and internal organs samples. Also, LSDV could be detected in CAMs of ECEs using PCR at first day Post-inoculation (PI) and by IF and IP at second day post-inoculation before appearance of characteristic pock lesions on CAM. © 2011 Academic Journals Inc.