Molecular survey and genetic diversity of Plasmodium sp. infesting domestic poultry in northeastern Thailand

Abstract

Introduction: Haemosporidian parasites are prevalent worldwide and can cause economic losses in poultry production. These parasites are arousing interest in Thailand and are found in many avian species. There is insufficient information on the genetic diversity of these alveolates from the largest families - Plasmodidae, Haemoprotidae and Leucocytozoidae - specifically parasitising ducks, turkeys, and geese. Material and Methods: Blood samples from 116 backyard poultry (60 ducks, 36 turkeys and 20 geese) in northeastern Thailand were investigated for Plasmodium spp., Haemoproteus spp. and Leucocytozoon spp. infections using microscopic examination and molecular approaches. Results: A total of 37/116 birds (31.9%) had confirmed Plasmodium infections. The prevalence was 69.4% (25/36) in turkeys, 18.3% (11/60) in ducks, and 5.0% (1/20) in geese. Of these 37 positives, 86.5% were Plasmodium sp., 10.8% were P. gallinaceum and 2.7% were P. juxtanucleare. Sequence analysis based on the cytochrome b gene identified seven lineages, of which two were new lineages in backyard poultry. Conclusion: This is the first report on the prevalence of haemosporidian parasites in backyard poultry in northeastern Thailand. The results provide important data for better understanding the molecular epidemiology of haemosporidian parasites infection in poultry in this region, which will be helpful in controlling these blood parasites.