Microbial biofilms produced by pseudomonas fluorescens on solid surfaces

Abstract

A biofilm is a complex aggregation of microorganisms growing on a solid substrate. Biofilms are characterized by structural heterogeneity, genetic diversity, complex community interactions, and an extracellular matrix of polymeric substances. The experimental part was focused on the adhesion of bacterial cells under static conditions and testing the effectiveness of disinfectants on created biofilm. In laboratory conditions we prepared and formed the bacterial biofilms Pseudomonas fluorescens in the four test surfaces of stainless steel, glass and plastic materials - PE (polyethylene) and EPDM (ethylene propylene diene monomer). Over the next 72 hours and 72 hours were observed numbers of adhesion bacterial cells of P. fluorescens on solid surfaces of tested materials. The highest values adhesion cells reached P. fluorescens cells after 72 hours of cultivation on plastic surfaces, where  was increased in adhesion bacterial cells for EPDM in the values of 105 CFU/cm2 and for PE up to 106 CFU/cm2. The subsequent repeated 72-hour cultivation P. fluorescens was an increase (growth) in the number of adhesion bacterial cells to all tested surfaces.