Specific detection of Pyricularia oryzae pathotype Triticum using qPCR and LAMP methods

Abstract

Wheat blast is an important disease that affects cross-border food security. The causal pathogen, Pyricularia oryzae (syn. Magnaporthe oryzae) Triticum pathotype (MoT), forms a phylogenetic group distinct from the other P. oryzae pathotypes pathogenic on various gramineous plants. Here we developed MoT-specific primer sets for qPCR and LAMP detection methods, and their amplification specificity and efficiency were tested in comparison with previous reported MoT-specific primers. The amplification efficiency and specificity were affected by the polymerase premix. To evaluate the detection specificity, purified DNA samples of 15 MoT isolates, 89 non-MoT isolates of Pyricularia spp., and crude DNA from wheat plants inoculated with MoT were used. We conclude that the Sp3 primer sets for qPCR and the MoT3L primer sets for LAMP were the best in this research.