Capture of dengue virus type 3 using anionic polymer-coated magnetic beads

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Abstract

Dengue virus (DENV) is a mosquito-borne virus and can be transmitted to humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes is the most effective way of controlling DENV infections, detection of DENVs in mosquitoes is limited by the low sensitivity of available detection methods. We here report a method for capturing DENV type 3 (DENV-3) from mosquito cells using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). The beads were incubated with cell culture medium of DENV-3-infected mosquito cells, then separated from the supernatant by applying a magnetic field and washed. Adsorption of DENV-3 on the beads was confirmed by reverse transcription-polymerase chain reaction, which detected the presence of 3 genomic RNA on the beads, and Western blotting, which determined the major DENV-3 envelope protein on the beads. Therefore, this capture method may enable an improvement in DENV-3 detection.

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APA

Sakudo, A., Masrinoul, P., Tanaka, Y., & Ikuta, K. (2011). Capture of dengue virus type 3 using anionic polymer-coated magnetic beads. International Journal of Molecular Medicine, 28(4), 625–628. https://doi.org/10.3892/ijmm.2011.733

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