Simplification of lipase design in the enzymatic kinetic resolution of amines by saturation transfer difference NMR

Abstract

In this work, we demonstrate a nuclear magnetic resonance (NMR) method for racemic amide and lipase interaction as a first-pass design method in the enzymatic kinetic resolution of amines. As a novel adaptation of commonly used protein-ligand screening NMR methodologies, this approach relies upon a lipase-Amide interaction wherein the time-consuming is reduced drastically and new insights are produced during the development of biocatalysis reactions.