Chemical characterization of constituents isolated from hamelia patens and investigating its cytotoxic activity

Abstract

SRB cytotoxicity assay was performed for different extracts of Hamelia patens Jacq. family Rubiaceae; crude flowers (CF), crude leaves (CL), chloroform (Chl.L) and methanol (Me.L) fractions from leaves against liver (HepG-2) and breast (MCF-7) human carcinoma cell lines. Results demonstrated potent cytotoxic action against HEPG-2 for CF, Chl.L and Me.L with IC50 = 47, 30 and 44.4 μg/mL, respectively. On the other hand, CF, CL, Chl.L and Me.L had exerted powerful effect against MCF-7 with IC50 = 23.8, 25.5, 17.7 and 64 μg/mL, respectively. Chromatographic investigation of Me.L , resulted in the isolation and spectroscopic identification of rutin (1), isoquercetin (2) and soyasaponin Bb (3), which was isolated for the first time from the plant leaves and was identified by UPLC/ITMS/MS analysis. Comparative study for using ordinary method versus green methods of extraction was also inspected.