Cardiac remodelling in rainbow trout Oncorhynchus mykiss Walbaum in response to phenylhydrazine-induced anaemia

Abstract

We examined the nature, extent and timing of cardiac ventricular remodelling in response to chronic, chemically induced anaemia in warm- and cold-acclimated rainbow trout Oncorhynchus mykiss. Chronic anaemia was induced by bi-weekly injections of phenylhydrazine hydrochloride (PHZ) and resulted in transient but large decreases in haematocrit (Hct) and haemoglobin concentration. After 2 weeks of anaemia, relative ventricular mass (rM v) in warm-acclimated rainbow trout had already increased significantly and, by the eighth week of anaemia, rMv was 58% greater than in the sham-injected control fish. Temperature modulated the anaemia-induced ventricular remodelling and erythropoietic responses, as indicated by cold-acclimation reducing the extent of the cardiac remodelling and slowing erythropoietic recovery. For example, in cold-acclimated fish, PHZ reduced Hct to 8.8±1.9% (ranging from 4-16%) and increased rMv by 15% over a 4-week period, whereas the same treatment in warm-acclimated fish reduced Hct to only 17.4±2.1% (ranging from 6-29%) and yet increased rMv by 28%. Cold-acclimated fish also recovered more slowly from anaemia. In addition, warm-acclimated fish maintained compact myocardium between 32% and 37% during anaemia, while cold-acclimated fish responded with an increase in compact myocardium (from 29% to 37%). Routine cardiac output (Q̇) was continuously monitored following a single PHZ injection to examine the initial cardiac response to anaemia. Contrary to expectations, acute anaemia did not produce an immediate, proportionate increase in routine Q̇. In fact, Q̇ did not increase significantly until Hct had decreased to 10%, suggesting that rainbow trout may initially rely on venous oxygen stores to compensate for a reduced arterial oxygen-carrying capacity. Thus, we conclude that myocardial oxygenation, acclimation temperature and cardiac work load could all influence anaemia-induced cardiac remodelling in rainbow trout.