Polarization image segmentation of radiofrequency ablated porcine myocardial tissue

Abstract

Optical polarimetry has previously imaged the spatial extent of a typical radiofrequency ablated (RFA) lesion in myocardial tissue, exhibiting significantly lower total depolarization at the necrotic core compared to healthy tissue, and intermediate values at the RFA rim region. Here, total depolarization in ablated myocardium was used to segment the total depolarization image into three (core, rim and healthy) zones. A local fuzzy thresholding algorithm was used for this multi-region segmentation, and then compared with a ground truth segmentation obtained from manual demarcation of RFA core and rim regions on the histopathology image. Quantitative comparison of the algorithm segmentation results was performed with evaluation metrics such as dice similarity coefficient (DSC = 0.78 - 0.02 and 0.80 - 0.02), sensitivity (Sn = 0.83 - 0.10 and 0.91 - 0.08), specificity (Sp = 0.76 - 0.17 and 0.72 - 0.17) and accuracy (Acc = 0.81 - 0.09 and 0.71 - 0.10) for RFA core and rim regions, respectively. This automatic segmentation of parametric depolarization images suggests a novel application of optical polarimetry, namely its use in objective RFA image quantification.