Characteristics and bioactivity of a chlorogenic acid-producing endophytic bacterium isolated from Lonicera japonicae

Abstract

In present study, endophytes were isolated from medicinal plant extract of Lonicera japonicae for presence of chlorogenic acid (CGA) by HPLC and LC-MS. An endophytic baterium RB2, identified as Bacillus subtilis, was confirmed to produce chlorogenic acid and isomer neochlorogenic acid. Cinnamic acid and p-coumaric acid, two intermediates of CGA synthetic pathway, were detected in RB2 extract. The results suggested that RB2 may produce CGA in similar synthetic pathway to its host plant. The RB2 extract also exhibited certain antimicrobial activity with strongest effect against Pseudomonas aeruginosa and Candida albicans, and antioxidant activity with certain DPPH scavenging and FRAP value. RB2 extract displayed stability at low pH and high temperature, as for chlorogenic acid. The RB2 can be hopeful as the potential alternative source of CGA in future.