Endophyte bacteria alter physiological traits and promote growth of rice (Oryza sativa L.) in aluminium toxic and phosphorus deficient acid inceptisols

Abstract

Endophytic bacteria are known to impart biotic and abiotic stress tolerance under stressful environments. More than 70% arable area in North-Eastern Himalaya is occupied by acidic soil. The aim of this research was to test the extent of endurance achieved by suitable. Endophytic bacterial inoculation for enhancing crop fitness and also to quantify the realistic benefits for crop growth and performance with bacterial inoculation grown in acidic soil. To achieve this, the strain 22WE, isolated from the wild rice variety Zizania latifolia, was selected because of its growth promoting properties. 16S rDNA sequence of the isolate confirmed its identification as Serratia nematodiphila. This study assessed whether endophyte inoculation can provide the habitat-fitness benefits to rice crops in acidic soil. Further, Kharif rice (var. CAUR3) plants were inoculated with the endophyte selected strain of Serratia nematodiphila. Endophyte inoculated rice plants had significantly (P<0.05) higher root surface area, root volume, root and shoot biomass contents than that of uninoculated plants grown under aluminium (Al) toxic and phosphorus (P) nutrient conditions. The DAB staining assay exhibited that endophyte inoculation confers advantage to rice plants in terms of reduced reactive oxygen against Al-induced stress. The interaction effects between endophyte inoculation and Al levels was significant (P<0.05) for the malondialdehyde and cell membrane stability values. The current study showed that toxic effect of an approximate 100ppm Al incurred under any acid soil can be compensated due to the endophyte inoculation.