Neurotoxicity of pre-incubated alpha-synuclein with neutral nanoliposomes on PC12 and SHSY5Y cell lines

Abstract

Alpha-synuclein (α-Syn) is a protein with high tendency to convert into toxic aggregates that are involved in Parkinson's Disease (PD). The conversion of α-Syn into toxic aggregates may associate with bio-membranes and the interaction between α-Syn and bio-mimic liposomes can trigger the neurotoxicity. Some inhibitors of α-Syn aggregation are challengeable drugs. Using nano carriers such as nanoliposomes may overcome such challenges. Nevertheless, there are few studies on nanoliposomes effects on neuronal cells and α-Syn. Herein, we investigated the neurotoxicity and fibrinogenesis of neutral charged nanoliposomes. Thin layer evaporation method and hydration method was employed to formulate the nanoliposomes from Dipalmitoylphosphatidylcholine to the size of ≤ 100 nm. Fibrillation and cytotoxicity of α-Syn treated with nanoliposome were measured. Neither neurotoxicity nor fibrillation was observed when α-Syn treated with different concentrations of nanoliposome. These results well indicate that nanoliposome at the concentrations required for drug delivery not only is able to prevent fibrillation process, but also has no considerable toxic effects on the cells.