Efeitos do agaricus blazei na clastogenicidade induzida pela radiação ultravioleta em cultura de células CHO-K1

Abstract

The Agaricus blazei Murril ss. Heinemann (ABM), an edible mushroom native from Brazil, has been used in popular medicine on the treatment of several diseases, including cancer. The purpose of the present study was to evaluate the effects of the ABM extract (0.4%) on the clastogenicity induced by the exposure to ultraviolet radiation (UV), in CHO-K1 culture cells, through the chromosome aberration test. The cells were treated in different conditions (continuous treatment, pre-treatment and posttreatment) associated to DNA damage induction by UV. The analysis of the data demonstrated that the UV and the ABM present clastogenic activity. On pre and post-treatment protocols, anticlastogenic effects have not been evidenced. However, the continuous treatment protocol presented 86.1% damage reduction, a protective effect. The results do not clarify about the mechanism of action of the ABM extract, which could act either through dysmutagenesis or through bioantimutagenesis. Nevertheless it is evident its effect on the reduction of damage caused by non-ionizing radiation, in spite of presenting clastogenic activity at very high concentration.