Synergistic properties of cellulases from Clostridium cellulovorans in the presence of cellobiose

7Citations
Citations of this article
25Readers
Mendeley users who have this article in their library.

Abstract

An anaerobic mesophile, Clostridium cellulovorans, produces a multienzyme complex called the cellulosome and actively degrades polysaccharides in the plant cell wall. C. cellulovorans also changes cellulosomal subunits to form highly active combinations dependent on the carbon substrate. A previous study reported on the synergistic effects of exoglucanase S (ExgS) and endoglucanase H (EngH) that are classified into the glycosyl hydrolase (GH) families 48, and 9, respectively. In this study, we investigated synergistic effects of ExgS and EngK, a GH9 cellulase different from EngH. In addition, since EngK was known to produce cellobiose as its main product, the inhibition on cellulase activity of EngK with cellobiose was examined. As a result, the effect of cellobiose inhibition on EngK coexistent with ExgS was found to be much lower than that with EngH. Thus, although EngH and EngK are in the same GH9 family, enzymatic activity in the presence of cellobiose was significantly different.

Cite

CITATION STYLE

APA

Yamamoto, K., & Tamaru, Y. (2016). Synergistic properties of cellulases from Clostridium cellulovorans in the presence of cellobiose. AMB Express, 6(1), 1–6. https://doi.org/10.1186/s13568-015-0169-5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free