STUDY OF UV MUTATED PROTEASE ENZYME FROM VARIOUS SOURCES

Abstract

Proteases have wide variety of functions and commercial applications. Protease is the third largest groups of industrial enzymes and is used in detergents production, leather industry, food industry, pharmaceutical industry and bioremediation processes. Protease and amylase are well known industrially important enzymes. To study the UV mutated protease enzyme from various sources. Microbes were isolated from curd, buccal cavity are probiotics were maintained under optimum conditions, the microbial growth and enzyme production carried out at 37°C for at 24 hours and pH 7.0. The isolated microbes were mutated using UV radiation exposure which enhanced protease and amylase enzymes production. The protease enzyme was confirmed using protease and amylase assay. The isolated bacteria have the ability to produce the industrially valuable enzymes which have various industrial applications. The protease enzyme has wide applications in many industries and therefore the UV mutated enzyme will have more thermostability.