Amino acids and fatty acids profiles of eel (Monopterus albus) water extracts

Abstract

Eel (Monopterus albus) is containing amino acids and fatty acids that important to nutrition and medication. The objective of this study is to investigate the amino acids and fatty acids content of Monopterus albus water extracts. Amino acids contents were analyzed using precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) method. Fatty acids in Monopterus albus water extracts were esterified using transesterification method to form FAMEs before analyzed using GC. Boron trifluoride-methanol reagent was used for transesterification. The result of the study showed that Monopterus albus water extracts contain 15 kinds of amino acids and 9 kinds of fatty acids. The major amino acids in the extract are glycine (5.26 mg/g), alanine (4.39 mg/g), phenylalanine (2.16 mg/g), lysine (1.88 mg/g), proline (1.84 mg/g) and arginine (1.73mg/g). While the major fatty acids in the extract are oleic acid (192.23 µg/5 g), palmitic acid (184.48 µg/5 g) and pentadecanoic acid (154.03 µg/5 g).