Sufficient Light Intensity Is Required for the Drought Responses in Sweet Basil (Ocimum basilicum L.)

Abstract

Various environmental factors not only affect plant growth and physiological responses individually but also interact with each other. To examine the impact of light intensity on the drought responses of sweet basil, plants were subjected to maintenance of two substrate volumetric water contents (VWC) using a sensor-based automated irrigation system under two distinct light intensities. The VWC threshold was set to either a dry (0.2 m3·m−3) or sufficiently wet condition (0.6 m3·m−3) under low (170 μmol·m−2·s−1) or high light intensities (500 μmol·m−2·s−1). The growth and physiological responses of sweet basil (Ocimum basilicum L.) were observed over 21 days in the four treatment groups, where the combination of two environmental factors was analyzed. Under high light intensity, sweet basil showed lower Fv/Fm and quantum yield of PSII, compared to that under low light intensity, regardless of drought treatment. Fourteen days after drought treatment under high light intensity, stomatal conductance and the photosynthetic rate significantly reduced. Whereas plants under low light intensity showed similar stomatal conductance and photosynthetic rates regardless of drought treatment. Assessment of shoot and root dry weights revealed that plant growth decline caused by drought was more pronounced under high light intensity than under low light intensity. Thus, sweet basil showed significant declines in growth and physiological responses owing to drought only under high light intensity; no significant changes were observed under low light intensity.