Radioimmunoassay of free light chains of immunoglobulins in urine

Abstract

We describe radioimmunoassays for kappa and lambda light chains of immunoglobulins in urine. The assays, which involve antisera to free light chains, were sufficiently sensitive to measure the light chains in unconcentrated urine from healthy subjects. The usefulness of the assays in clinical practice is illustrated by measurements of light chain excretion by patients, including serial studies on patients undergoing treatment for myeloma.