Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803

Abstract

We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steadystate fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP. © 2013, American Society for Microbiology.