Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities

Abstract

The mechanism of U6 small nuclear ribonucleoprotein (snRNP) biogenesis is not well understood. Here the authors characterize the enzymatic activities and structures of yeast and human U6 RNA processing enzyme Usb1, reconstitute post-Transcriptional assembly of yeast U6 snRNP in vitro, and propose a model for U6 snRNP assembly.