Relaxation activity of tetanus (Leea aequata L.) leaf ethanolic extract on Guinea pig isolated trachea

Abstract

Objective: The aim of this study is to determine the relaxation activity of the ethanolic extract of tetanus leaf (EETL) against contracting isolated guinea pig trachea induced by acetylcholine. Methods: Isolated trachea was equilibrated for 45 min until a stable condition in the Creb’s solution with a temperature of 37°C aerated with carbogen gas (O2:CO2=95%:5%). Test of the relaxation effect conducted after guinea pig trachea contracted with maximum acetylcholine concentration (EC80: 2.0449x10-3 mol) and then a cumulative with concentration of EETL (0.5 mg/mL-4 mg/mL) and concentration of atropine sulfate (6.95x10-6 mg/mL-2.08x10-2 mg/mL). Results: The results showed that EETL could lower the contraction smooth muscle of guinea pig trachea induced by acetylcholine. EETL at a concentration of 3.5 mg/mL had the ability to relax the muscle. It was not significantly different compared to that of atropine sulfate 6.95x10-3 mg/mL (p>0.005). Conclusion: EETL has a relaxation effect on smooth muscle of guinea pig isolated trachea.