The polymorphisms of goat THRSP gene associated with ecological factors in Chinese indigenous goat breeds with different Lipogenesis ability

Abstract

THRSP gene has been regarded as a candidate gene for lipogenesis in domestic animals. To understand the association of THRSP gene with habitats and fatty deposits in goat breeds, the polymorphisms of goat THRSP gene, the fat content of goat carcass and their association with the ecological factors were investigated. The Chinese indigenous goat breeds selected were Guishan, Yunling, Chuandong, Banjiao, Xiangdong, Matou and Anhui white goat. The polymorphisms in the 5' regulatory region and exon 1 of THRSP gene were analyzed using PCR-RFLP. The longissimus Muscle Fat Grade (MFG) and Carcass Fat Grade (CFG) of all goat breeds were estimated. The observed data of ecological factors were provided by the meteorological observatory in the goat habitats which included Average Daily Rainfall (ADR), Elevation (E), average Daily Sunshine Duration (DSD), Latitude (LAT) and longitude. E/LAT, ADRxDSD and SCxEC were regarded as Space Coefficient (SC), Energy Coefficient (EC) and Ecological Index (EI), respectively. The results showed that three mutational sites, C-233T, G113A and A138G, were detected. Nine genotypes in all goat breeds were only found, of which the frequencies of TCAAGG, TTGAAG and TTAAGG genotype were lower than 0.05. The EC significantly related to CAG haplotype frequency (0.8859, p<0.01) and the associations of EI with TGA haplotype frequency was significant (-0.8377, p<0.025). In the four haplotype frequencies, the relationship between CAG haplotype frequency and MFG was significant (-0.8633, p<0.025). The results suggest that the polymorphism of goat THRSP gene may be associated with the ecological factors and affect the goat lipogenesis ability.