Involvement of β-adrenergic receptor in synaptic vesicle swelling and implication in neurotransmitter release

Abstract

Secretory vesicle swelling is required for vesicular discharge during cell secretion. The Gαo-mediated water channel aquaporin-6 (AQP-6) involvement in synaptic vesicle (SV) swelling in neurons has previously been reported. Studies demonstrate that in the presence of guanosine triphosphate (GTP), mastoparan, an amphiphilic tetradecapeptide from wasp venom, activates Go protein GTPase, and stimulates SV swelling. Stimulation of G proteins is believed to occur via insertion of mastoparan into the phospholipid membrane to form a highly structured α-helix that resembles the intracellular loops of G protein-coupled adrenergic receptors. Consequently, the presence of adrenoceptors and the presence of an endogenous β-adrenergic agonist at the SV membrane is suggested. Immunoblot analysis of SV using β-adrenergic receptor antibody, and vesicle swelling experiments using β-adrenergic agonists and antagonists, demonstrate the presence of functional β-adrenergic receptors at the SV membrane. Since a recent study shows vH+-ATPase to be upstream of AQP-6 in the pathway leading from Gαo-mediated swelling of SV, participation of an endogenous β-adrenergic agonist, in the binding and stimulation of its receptor to initiate the swelling cascade is demonstrated. © 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.