Structural and functional characterization of VanG D-Ala:D-Ser ligase associated with vancomycin resistance in Enterococcus faecalis

Abstract

Background: D-Ala:D-Lac and D-Ala:D-Ser ligases are key enzymes in vancomycin resistance. Results: The VanG D-Ala:D-Ser ligase structure provided insight into its molecular specificity and allowed the selection of a specific peptide inhibitor. Conclusion: D-Ala:D-Lac and D-Ala:D-Ser ligases share specific determinants. Significance: This study sheds light on the molecular specificity and evolution of D-Ala:D-X ligases and could help in designing inhibitors to overcome vancomycin resistance. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.