Caracterização das lesões por Cysticercus bovis, na inspeção post mortem de bovinos, pelos exames macroscópico, histopatológico e pela reação em cadeia da polimerase (PCR)

Abstract

Considering the importance of improving methods for diagnosis of bovine Cysticerco- sis, this study aimed to verify Cysticercus bovis occurrence in different anatomical sites, as head, heart, esophagus, diaphragm, tongue, liver and carcass, examined by federal inspec- tion service. Diagnosis was performed by gross examination, histopatholgy and PCR with boiling DNA extraction for metacestode identiCambria.-1.g976ication. Of 22043 slaughtered cattle, 713 (3.23%) were infected. The heart was mostly affected with 1.90% (420/22043), followed by head, 1.11% (245/22043), esophagus, 0.08% (18/22043), carcass, 0.07% (15/22043), diaphragm, 0.03% (7/22043), liver, 0.02% (5/22043) and tongue, 0.01% (3/22043). Of the cysts obtained, 58.35% (416/713) were dead and 41.65% (297/713) were alive. The differences among anatomical sites and cysts status were signiCambria.-1.g976icant (p<0.05). Of the 416 dead cysts 253, characterized by nodular Cambria.-1.g976irm whitish lesions, containing yellowish mate- rial, some times in calcareous aspect were examined for histopathology. The histological exams of these cysts yielded granulomatous lesions, whose centers were characterized by caseous and/or calcareous material, multinucleate giant cells, histiocytes in palisade and inCambria.-1.g976iltrate composed predominantly by lymphoid cells, wrapped up by Cambria.-1.g976ibrosis. Some times the lesions peripheries had granulation tissue and mineralized areas, like linear blade. The parasite debris were like a hyaline, non cellular material with spherical and ovoid, baso- philic, eosinophilic and colorless corpuscles. These corpuscles were seen rarely, some ti- mes, among inCambria.-1.g976lammatory reaction. Fibrous nodules, rich in lymphoid or mixed inCambria.-1.g976iltrates, were frequently seen. Of the live cysts subjected to PCR with boiling DNA extraction, 65% (13/20) were positive for C. bovis, conCambria.-1.g976irming the ambulatory diagnosis and the efCambria.-1.g976icacy of the PCR procedure used. Due to microscopic and PCR diagnostic exams of C. bovis, mainly in the liver and esophagus, it is suggested changes in the 176 article of the regulatory ins- pection, by including these sites in the bovine routine inspection at the slaughterhouses.