Effect of cigarette smoking on pentane excretion in alveolar breath

Abstract

The concentrations of acetone, isoprene, and pentane in alveolar breath were examined in 50 smokers and 50 non-smokers by gas chromatography. The baseline pentane in smokers was 0.17 ± 0.03 nmol/L (mean ± SE), which was not different from pentane in nonsmokers (0.23 ± 0.03 nmol/L). There were also no differences between smokers and nonsmokers in the concentrations of acetone and isoprene. Serial breath samples were obtained from 15 smokers before smoking and at 5, 15, and 60 min after smoking. Although acetone was not altered by smoking, isoprene increased by 86% ± 26% 5 min after smoking (P <0.001) and returned to baseline 10 min later. Pentane increased by 456% ± 156% 5 min after smoking (P <0.001) and remained increased 10 min later (204% ± 73% of baseline, P <0.05). Isoprene concentrations in mainstream cigarette smoke were >5000 times greater than breath concentrations, whereas pentane could not be detected in mainstream smoke. Because pentane is produced from the peroxidation of n-6 polyunsaturated fatty acids, the results provide evidence that cigarette smoking causes an immediate increase in lipid peroxidation.