A variety of anti-inflammatory agents have been shown to exert chemopreventive activity via targeting of transcription factors such as NF-κB and AP-1. Lithospermum erythrorhizon (LE) has long been used in traditional oriental medicine. In this study, we demonstrated the inhibitory effects of LE extracts on lipopolysaccharide (LPS)-stimulated production of inflammatory cytokines. As an underlying mechanism of inhibition, LE extracts reduced LPS-induced transactivation of AP-1 as well as NF-κB in mouse macrophage cells. Electrophoretic mobility shift assays indicated that LE extracts inhibited the DNA binding activities of AP-1 and NF-κB. In addition, phosphorylation of IκB-α protein was suppressed by LE extracts. Moreover, LE extracts inhibited c-Jun N-terminal kinase and extracellular signal-regulated signaling pathways. Our results suggest that the anti-inflammatory activity of LE extracts may be mediated by the inhibition of signal transduction pathways that normally lead to the activation of AP-1and NF-κB. These inhibitory effects may be useful for chemoprevention of cancer or other chronic inflammatory diseases.
CITATION STYLE
Kyu, Y. H., Taek, H. K., Tae, H. L., Lee, S. J., Kim, S. H., & Kim, J. (2008). Suppressive effects of Lithospermum erythrorhizon extracts on lipopolysaccharide-induced activation of AP-1 and NF-κB via mitogen-activated protein kinase pathways in mouse macrophage cells. Journal of Biochemistry and Molecular Biology, 41(4), 328–333. https://doi.org/10.5483/bmbrep.2008.41.4.328
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