Detection of the Inhibitory Effect of the Leaves, Seed and Fruits of Cydonia oblonga on some Gram Positive and Negative Bacteria

Abstract

Seventy six swab samples were collected from Otitis media and stool samples from gastroenteritis cases, were cultured on different culture media, gram positive and gram negative bacteria were isolated and identified. The highest prevalence was Staphylococcus aureus (25%) while Salmonella typhi formed the lowest isolate rate (3.9%) other bacterial isolates varied between these two and comparison was done between effect of the air part of Cydonia oblonga (Quince) plant (leaves, seeds, fruits) to determine the highest effective part on the isolated bacteria. As the inhibitory effect of Cydonia oblonga seed, leaves and fruit alcoholic and aqueous extracts on the isolated bacteria were studied. Alcoholic seed extract showed the highest inhibitory activity on two bacterial species Enterobacter aerogenes and Enterococcus faecalis (25 and 22 mm) respectively followed by Aeromona sp. and Pseudomonas aeruginosa (13 and 11 mm) respectively while alcoholic leaves extract showed antibacterial effect on Staphylococcus aureus only (15 mm), alcoholic fruit extract showed its effect on Enterococcus faecalis (12 mm), Klebsiella sp. (11 mm), Salmonella typhi (10 mm), and Pseudomonas aeruginosa (9 mm). the aqueous seed extract showed an effect on Enterococcus faecalis (11 mm), while the aqueous leaves extract showed the highest effect on Staphylococcus aureus (20 mm), followed by Bacillus subtilis (10 mm), and the aqueous fruit extract showed the highest effect on E.coli (10 mm) while on Enterococcus faecalis, Klebsiella sp. and Enterobacter aerogenes was less (9 mm) for all of them. The (MIC) value of the effective extracts revealed that the highest value of alcoholic seed extract on E.faecalis was (25 mg\ml), while the least (MIC) was (0.39 mg\ml) for Aeromona sp. with the remaining MIC values ranging between the two.