One-year stability of glucose dehydrogenase confined in a 3D carbon nanotube electrode with coated poly-methylene green: Application as bioanode for a glucose biofuel cell

Abstract

We report a new approach to fabricate an efficient 3D glucose bioanode based on the co-immobilization of the enzyme glucose dehydrogenase (GDH), its cofactor NADP, and Multiwall Carbon Nanotubes (MWCNTs) coated with poly (methylene green) (PMG). The MWCNT-PMG composite was obtained by chemical polymerization of methylene green (MG) monomer on the MWCNT surfaces. Structural and chemical analyses clearly showed successful coating of the MWCNTs with PMG that markedly affected their morphological and surface charge properties. Electrochemical investigation of PMG-MWCNTs mixed with GDH and NADP showed high stability with extended bioanode electrocatalytic activity toward glucose oxidation for more than one year.