Elastase inhibitory activity of methanol extract and n-hexane extract of Garcinia xanthochymus Pericarp

Abstract

Garcinia xanthochymus is a plant found in Indonesia and rich in quercetin. Ethanol extract of bark can reduce LDL levels. Xanthon from the G. xanthochymus has antidiabetic activity. This study aims to determine the anti-elastase activity of n-hexane extract and methanol extract of G. xanthochymus pericarp. Samples were macerated for one time 24 hours using n-hexane and methanol, respectively. The elastase enzyme inhibition test used spectrophotometry methods by reacting extracts with the porcine pancreatic elastase enzyme and using the substrate N-Succ-(Ala)-3-p-nitroanilide (SANA). Absorbance was measured at a wavelength of 405 nm with a microplate reader. The results showed that the concentration of 100 ppm extract of n-hexane extract of pericarp had enzyme inhibiting activity 65.17 ± 6.44%, methanol extract of pericarp 29.81 ± 10.67%. Meanwhile, the inhibitory activity of quercetin elastase enzyme as a positive control was 62.75 + 1.89%. This research concludes that the n-hexane extract of the pericarp of G. xanthochymus has activity as an inhibitor of the enzyme elastase is very high and can potentially be used as a material for making cosmetics.