An immunochromatography test strip for rapid, quantitative and sensitive detection of furazolidone metabolite, 3-amino-2-oxazolidinone, in animal tissues

Abstract

3-amino-2-oxazolidinone (AOZ) is the metabolite of furazolidone, which has been banned as a veterinary drug due to the potential harmful effects on human health. In this study, a novel immunochromatography test strip (ICTS) based on colloidal gold–McAb probe for detection of AOZ within tissue samples was developed. With this method, the visual cut-off levels for the test strip was achieved at 10 μg/L. Using a test strip reader, the 50% inhibition was 1.3 μg/L. The limit of detection and limit of quantification in four kinds of animal tissues were 0.15 μg/kg and 0.31 μg/kg, respectively. When used to analyze fortified samples of animal tissue, acceptable recovery rates of 76.3–98.4% were obtained. The fortified samples were detected by ICTS and confirmed by LC-MS/MS. The results of the two methods were in good agreement. The proposed ICTS was demonstrated to be a rapid and simple analytical method for detecting AOZ in animal tissue.