Application of micellar electrokinetic capillary chromatography to the determination of flavonoid drugs

Abstract

The use of micellar electrokinetic capillary chromatography is described for the determination of flavonol-3-O-glycosides. A 72-cm fused-silica capillary column and a 50 mM sodium dodecyl sulphate-20 mM sodium borate running buffer (pH 8.3) were used for the electrophoretic separations. The samples were injected onto the capillary column in the range 10-1000 pg. Results are given for quercetin-, kaempferol- and isorhamnetin-3-O-glycosides and for a standardized Ginkgo biloba extract. The results are compared with those obtained by reversed-phase high-performance liquid chromatography. © 1991.