Methodologies for determining the specific methanogenic activity (SMA) in anaerobic sludges

Abstract

This paper presents a review on the different methodologies to determine the specific methanogenic activity (SMA) of anaerobic sludges. The protocols available in the literature differ not only regarding the procedures adopted to incubate the sludge, but also in relation to the method utilized to quantify the methane produced during the test. This paper discusses the principles of manometric and volumetric methods, and briefly describes the protocols mostly used by the Brazilian research community for sludge incubation, methane measurement and calculation of SMA.