Implications of reconstruction protocol for histo-biological characterisation of breast cancers using FDG-PET radiomics

Abstract

Background: The aim of this study is to determine if the choice of the 18 F-FDG-PET protocol, especially matrix size and reconstruction algorithm, is of importance to discriminate between immunohistochemical subtypes (luminal versus non-luminal) in breast cancer with textural features (TFs). Procedures: Forty-seven patients referred for breast cancer staging in the framework of a prospective study were reviewed as part of an ancillary study. In addition to standard PET imaging (PSF WholeBody ), a high-resolution breast acquisition was performed and reconstructed with OSEM and PSF (OSEM breast /PSF breast ). PET standard metrics and TFs were extracted. For each reconstruction protocol, a prediction model for tumour classification was built using a random forests method. Spearman coefficients were used to seek correlation between PET metrics. Results: PSF WholeBody showed lower numbers of voxels within VOIs than OSEM breast and PSF breast with median (interquartile range) equal to 130 (43–271), 316 (167–1042), 367 (107–1221), respectively (p < 0.0001). Therefore, using LifeX software, 28 (59%), 46 (98%) and 42 (89%) patients were exploitable with PSF WholeBody , OSEM breast and PSF breast , respectively. On matched comparisons, PSF breast reconstruction presented better abilities than PSF wholeBody and OSEM breast for the classification of luminal versus non-luminal breast tumours with an accuracy reaching 85.7% as compared to 67.8% for PSF wholeBody and 73.8% for OSEM breast . PSF breast accuracy, sensitivity, specificity, PPV and NPV were equal to 85.7%, 94.3%, 42.9%, 89.2%, 60.0%, respectively. Coarseness and ZLNU were found to be main variables of importance, appearing in all three prediction models. Coarseness was correlated with SUV max on PSF wholeBody images (ρ = − 0.526, p = 0.005), whereas it was not on OSEM breast (ρ = − 0.183, p = 0.244) and PSF breast (ρ = − 0.244, p = 0.119) images. Moreover, the range of its values was higher on PSF breast images as compared to OSEM breast , especially in small lesions (MTV < 3 ml). Conclusions: High-resolution breast PET acquisitions, applying both small-voxel matrix and PSF modelling, appeared to improve the characterisation of breast tumours.