HPLC determination of jolkinolide A and jolkinolide B in Chinese herb medicine＂ Lang- du＂

Abstract

Objective：To establish a method for the determination of two diterpenoide lactones,jolkinolide A and j olkinolide B in Chinese herbal medicine ＂Lang -du＂ by HPLC. Methods： C18 column（4.6 mm *250 mm,5 mum） was used with acetonitrile -water（75：25 ）as the mobile phase. The flow rate was 1.0 mL· min^-1 ,and the detection wavelength was 250 nm. Results： By using the established HPLC method, nine samples of ＂Lang - du＂ were analyzed to determine the contents of jolkinolide A and B. It was showed that the contents of jolkinolide A and B ranged from 0. 007% to 0. 043% and 0. 012% to 0. 204% , respectively. Among the samples, the two diterpenoids were found in most of the samples of Euphorbia fischeriana and E. ebracteolata, but Stellera chamaejasme and Alocasia macrorrhiza contained neither jolkinolide A nor jolkinolide B. Conclusion： A simple, effective and feasible HPLC method with good accuracy, precision and reproducibility to determine the contents of two diterpenoids in＂ Lang - du＂ was established which could be applied for the quality control of this herb. 目的：建立测定狼毒药材中2个二萜内酯类成分岩大戟内酯A和B的HPLC定量分析方法。方法：采用C18柱（4．6mm*250mm，5mum），乙腈- 水（75：25）8t脱，流速为1．0mL·min^-1，检测波长为250nm。结果：狼毒大戟和月腺大戟中普遍含有岩大戟内酯A和B，2个成分的含量 范围分别为0．007％-0．043％和0．012％。0．204％。瑞香狼毒和海芋中不含有这2个成分。结论：本方法专属性强，结果准确，简便可行，适 用于狼毒药材中岩大戟内酯A和B的含量测定。